Pbs is formulated without calcium and magnesium for rinsing chelators from the culture before. Incubate for 30 minutes with the abc reagent.
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Pbs (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
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Pbs is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation.
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